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Fig. 1. Intracellular heme synthesis in PC12 cells treated with succinyl acetone. Heme was extracted from PC12 cells treated with 0, 0.5, or 1 mM succinyl acetone (SA), or 2 µM N-methyl protoporphyrin IX (N-MP) for 24 h, and the amount of radiolabeled heme was measured exactly as described previously (17) .