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Fig. 3. The parameters of proliferation of A.1 and 1.3 EC cells. EC cells of both lines were seeded in the same densities (2.5 x 103 cells/cm2) and cultured under nondifferentiating conditions for 24, 48, 72, and 96 h, respectively, and then harvested for analyses. A, growth rates. Cells were lysed in SDS-containing buffer, and the total amounts of protein were used as a measure of cell quantities. Micrograms of total protein are shown on the Y axis. A representative result is shown. B, the distribution of cells in cell cycle phases. Cells were fixed in Vindelov’s solution, stained with propidium iodide, and analyzed using FACSCalibur equipped with ModFit 2.0 software. The percentages of cells in G1, S, and G2 phases are expressed as the means of three independent experiments; bars, SE.