Fig. 2. The effect of cell density on the expression of p27 in nondifferentiated EC cells. Cells of A.1 and 1.3 lines were grown under standard conditions for 6, 24, and 48 h, respectively. A, cells were harvested, and the proteins were extracted. Equal amounts of total protein of each cell line were separated on 10% SDS-PAGE and Western analyzed using anti-p27 antibody. B, cells were pictured at the time of harvesting to document their density on dish. Data are representative of at least three independent replicates.