Fig. 10. The effect of overexpression of p27 on the level of cyclin (cyc) D3 and vice versa in EC cells. Both A.1 and 1.3 EC cells were transfected with either p27 or cyclin D3 construct. EC cell clones that were found to overexpress the appropriate molecule were further grown under standard conditions, and Western blot was analyzed for the amount of potentially coregulated p27 or cyclin D3. A, EC cells overexpressing p27. B, EC cells overexpressing cyclin D3. C, negative control; EC cells transfected with empty vector. At least three independent overexpressing clones were analyzed for each molecule.