Fig. 1. Expression of p27 protein in nondifferentiated cells of several EC cell lines. Cells grown under standard conditions were harvested, and the proteins were extracted. Equal amounts of total protein of each cell line were separated on 10% SDS-PAGE and Western analyzed using anti-p27 antibody. To control the loading, the blot was reprobed using anti-mitogen-activated protein kinase antibody. Data are representative of at least three independent replicates.