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Fig. 9. Effect of 15d-PGJ2 treatment on the phosphorylation of eIF-2{alpha} in wild-type and PKR-null mouse embryo fibroblast cells. A, mouse embryo fibroblast cells, either wild type (Pkr+/+) or PKR-null (Pkr0/0), were treated for the indicated period of time with 10 µM 15d-PGJ2. Phosphorylated eIF-2{alpha} or {alpha}-tubulin protein levels were determined by Western immunoblot analysis. B, Western blots were quantified by scanning densitometry. Error brackets, SE for three independent experiments. {square}, control wild-type cells; {blacksquare}, wild-type cells treated with 15d-PGJ2; {circ}, control PKR-null cells; {bullet}, PKR-null cells treated with 15d-PGJ2.