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Fig. 8. A, effect of 15d-PGJ2 on phosphorylation of eIF-2{alpha} in MCF-7 cells. Cultures were treated with 10 µM 15d-PGJ2 (T), DMSO vehicle (C), or 100 µM sodium arsenite (A) for the indicated times. Protein extracts were prepared and subjected to Western blotting. Blots were probed with specific antibody for phospho-eIF-2{alpha} (top) and then reprobed with antibody for total eIF-2{alpha} (middle) and ß-actin (bottom). B, results of experiments presented in A quantified by scanning densitometry. Error brackets, range for duplicate determinations.