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Fig. 4. Northern blots depicting the effect of 10 µM 15d-PGJ2 on cyclin D1 and GAPDH mRNA levels. A, effect on the quantity of cyclin D1 mRNA in cells treated for 2–8 h with 15d-PGJ2. B, effect in cells treated for 1 h. The major (4 kb) species of cyclin D1 mRNA is shown. C, vehicle-treated controls; T, treated with 10 µM 15d-PGJ2 for the time periods indicated.