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Fig. 2. Polyacrylamide gel analysis of TRAP assay products. Display of TRAP assay PCR products from early cultures of the cell lines shown in Fig. 1 . Results for untreated (+) and heat denatured lysates (-) are shown for each cell line. Control lanes (CON) show products generated from the manufacturer’s positive control template (+) and from a lysate buffer blank (-). Six-nucleotide ladders generated by telomerase activity are apparent in the JJ, FS, hTERT, CS-6, CS8, CS-9, and CON lanes.