Fig. 9. Gastrin stimulates JNK activation through a PI3K-independent mechanism. After a 30-min preincubation with or without the indicated concentrations of wortmannin (A) or LY 294002 (B), CHO cells expressing the human CCKBR were stimulated for 3 min with 10 nM gastrin. Whole cell lysates were immunoblotted with an anti-phospho-Thr183 and -Tyr185 JNK antibody. Comparable amounts of proteins were detected in whole cell lysates of all of the above-mentioned experiments (data not shown).