Fig. 6. Role of p60Src in the p130Cas/CrkII complex formation induced by gastrin. CHO cells expressing the CCKBR (CHO-CCKB) and CHO-CCKB cells stably transfected with the CSK or the p60Src kinase-inactive mutant (SRC- clone 2) were stimulated for the time indicated with 10 nM gastrin. Proteins from cell lysates were prepared and immunoprecipitated (IP) with anti-phosphotyrosine (P-Tyr; A and C) or anti-CrkII (B and D) antibodies and then subjected to p130Cas immunoblotting. Comparable amounts of proteins were detected in whole cell lysates or immunoprecipitates of all of the above-mentioned experiments (data not shown).