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Fig. 6. Farnesylated, but not unprenylated, RhoA(63L) retains the ability to antagonize HIV-1 replication and gene expression. A, the ability of the RhoA(63L) protein to block the transient replication of HIV-1 was assessed by cotransfecting the HIV-1 provirus pNL4-3 with pcDNA3 expression plasmids encoding the indicated proteins in 293T cells. At 40–50 h after transfection, HIV-1 virions in the culture supernatant were measured by reverse transcriptase activity ({square}) or by quantification of infectious units ({blacksquare}). The relative HIV-1 replication is presented as a percentage of vector controls (100%). B, the ability of RhoA(63L) protein to inhibit gene expression was assessed by cotransfecting pNL4-3-Luciferase plasmid with pcDNA3 expression plasmids encoding the RhoA proteins in Jurkat T cells. The level of luciferase expression (relative light units) was measured 48 h after transfection. Data points are the means of three independent measurements; bars, SE. Data shown are representative of two independent experiments.