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Fig. 5. RhoA(63L)-induced migration of T47D human breast carcinoma cells is dependent on prenylation, but not specific prenylation. T47D cells stably expressing the indicated protein were analyzed for their ability to migrate through the pores of a transwell to the underside. Fold activation was determined by the number of migrating cells relative to the number of migrating vector-transfected cells. Western blot analyses with anti-HA epitope antibody was done to verify equivalent expression of RhoA(63L) protein in each cell population; although WT and CVLS versions of RhoA(63L) showed comparable levels of expression, the SLVL mutant consistently showed slightly higher levels of expression in this and other analyses (not shown). Data points are the means of two independent measurements; bars, SE. Data shown are representative of three independent assays.