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Fig. 4. Farnesylated, but not unprenylated, RhoA(63L) retains the ability to promote growth in low serum. NIH 3T3 cells stably expressing RhoA(63L)-WT, RhoA(63L)-CVLS, or RhoA(63L)-SLVL were plated at 103 cells/dish and allowed to adhere in DMEM supplemented with 10% calf serum. Untransformed cells stably transfected with the empty pZIP-NeoSV(x)1 plasmid (Vector) were included as a control. After 24 h, the medium was replaced with DMEM supplemented with 0.5% calf serum. Twenty-one days after plating, the cells were fixed and stained with 0.4% crystal violet (A) to better visualize the cells. On day 20, the cells were photographed (B) to demonstrate the different morphology of the cells.