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Fig. 4. Comparable inhibition of MCF-7 clonogenic growth by RAR{alpha}2 and RARß2. MCF-7 cells were transfected with 2 µg of pIRES-2-EGFP, pRAR{alpha}2-IRES-2-EGFP, or pRARß2-IRES-2-EGFP and then plated at low density in selection medium supplemented with charcoal-stripped serum and the indicated concentration of RA. A, crystal violet-stained cultures after 3 weeks in selection medium. For each plate, wells within the same column represent triplicates. B, densitometric evaluation of cell number in the cultures shown in A. Similar results to those shown were obtained in two additional experiments.