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Fig. 7. Labeling of mitotic LLC-Pk cells stably expressing green fluorescent protein-topoisomerase II{alpha} (A, D) with antibody to topoisomerase IIß (B, E) and with the DNA stain DAPI (C, F). The prophase cell (A–C) was fixed in room temperature methanol. The anaphase cell (D–F) was lysed with detergent and fixed in formaldehyde. Before immunolabeling, both cells were treated with DNase to partially digest the DNA and better expose topoisomerase IIß antigen.