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Fig. 3. Triple labeling of HeLa cells (A–C) and human keratinocytes (D–F) with antibody to topoisomerase II{alpha} (A, D) antibody to topoisomerase IIß (B, E) and with the DNA stain DAPI (C, F). Among the interphase cells in both cultures, all nuclei are labeled with antibody to topoisomerase IIß. A subset of the interphase cells (open arrows) does not show labeling for topoisomerase II{alpha}. Cells in M phase (closed arrows) show labeling of mitotic chromosomes with antibodies to topoisomerase II{alpha} and topoisomerase IIß. Bar, 5 µm.