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Fig. 3. Colocalization of Fas/CD95 with the lysosomal marker lamp-1 in Jurkat parental cells, and in the hyperproliferative sublines Jurkat-ws and Jurkat-hp. Fas was located by confocal microscopy by using the SM1/23 mouse mAb and FITC-conjugated antimouse antibody (green fluorescence, left panels). Lamp-1 was located using a polyclonal rabbit antibody and Cy3-conjugated antirabbit antibody (red fluorescence, middle panels). Fas staining images were superimposed with lamp-1 staining ones, as indicated, to show overlapping signals as yellow fluorescence (right panels). Magnification, x2000.