Click on image to view larger version.



Fig. 6. Role of growth factors in the activity of bryostatin-1. U937 cells were incubated for 120 h in the absence (Control) or presence of bryostatin-1 (10-8 M) along with neutralizing antihuman GM-CSF (0.1 mg/ml) and antihuman IL-3 (0.15 mg/ml) or nonbinding isotypic control antibodies and assayed for CD11b expression (A) and clonogenic growth (B). All results represent the means of three separate experiments; bars, SE. P < 0.001 was determined by the paired Student’s ttest for the comparison of bryostatin-1 + isotypic control antibodies and bryostatin-1 + growth factor blocking antibodies for both CD11b expression (A) and clonogenic growth (B).