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Fig. 2. Effects of bryostatin-1 and GM-CSF on cell surface antigen expression by AML cell lines. U937 (A) and HL60 (B) cells were incubated for 120 h in the absence (Control) or presence of bryostatin-1 (Bryo, 10-8 M) with or without GM-CSF (200 units/ml) and then assessed for the surface expression of CD11b and CD14 by flow cytometry. Results represent means of five separate experiments; bars, SE. Ps were determined by ANOVA for the comparison of all treatment groups for both CD11b and CD14 expression.