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Fig. 4. Constitutively active ErbB4 receptors do not induce growth in semisolid medium. FR3T3 cells that stably express the LXSN vector control, the constitutively active ErbB2 mutant (ErbB2*), wild-type ErbB4, or the constitutively active ErbB4 mutants (Q646C, H647C, and A648C) were seeded in semisolid medium at a density of 2 x 104 cells/ml in 60-mm dishes. The cells were incubated for 10 days, after which images were recorded by photomicroscopy. Images shown are representative of those obtained in three independent experiments.