Click on image to view larger version.



Fig. 2. Q646C, H647C, and A648C mutants exhibit increased in vitro kinase activity. Equal amounts of protein lysates (1000 µg) from PA317 cells that stably express wild-type ErbB4 or the ErbB4 mutants (Q646C, H647C, and A648C) were immunoprecipitated with an anti-ErbB4 rabbit polyclonal antibody. Lysates from PA317 cells that express the LXSN vector served as the negative control. Kinase reactions were performed on the immunoprecipitates in the presence of [{gamma}-32P]ATP. The products were resolved by SDS-PAGE. The gel was dried overnight and exposed to X-ray film for ~20 h to visualize the products of the kinase reactions.