Fig. 5. Effects of TGIF and mSin3 expression on TGFß-activated transcription. L17 cells were cotransfected with the A3-lux reporter and a FAST2 expression construct, together with TGIF (+, 25 ng; ++, 50 ng/well) or mSin3 (+, 100 ng/well) expression vectors as indicated. TGFß was added after 24 h, and luciferase activity was assayed after an additional 18 h. Activity is shown as the mean ±SD of triplicate transfections.