Fig. 2. Interaction of endogenous TGIF and mSin3. Protein complexes were precipitated from human lung A549 cells using antisera specific for TGIF or mSin3 or with the appropriate preimmune sera. Proteins were visualized by Western blotting for TGIF, mSin3, and HDAC1. The positions of molecular weight markers (kDa) are shown.