Table 1 GPIX promoter sequence mutations

Mutations of the GATA and Ets motifs were introduced into the GPIX promoter sequence using site-directed mutagenesis with the primer sequences as shown below. Sequences are in 5' -> 3' direction. Numbering indicates nucleotide position upstream of transcriptional start site. GATA and Ets binding sites are underlined. Mutated bases are shown in bold.

Wild-type GPIX GATA sequence 79-CTGCACTGGGGGGATAAGCCAGGC55
GPIX GATA mutation 79-CTGCACTGGGGGAATTCGCCAGGC55
Wild-type GPIX Ets sequence 54-ATTTTCATCACTTCCTTCCGCCCGCTCCCG24
GPIX Ets mutation 54-ATTTTCATCACATACCTGCGCCCGCTCCCG24