Fig. 8. MAPK inhibition by PD98059 does not affect Fli-1 binding activity. EMSA was performed using nuclear extracts from Dami cells pretreated with PD98059 or SB202190 (data not shown) and subjected to PMA stimulation in the presence of anti-Fli-1 antibody as outlined in "Materials and Methods." The specific oligonucleotide-Fli-1 complexes are indicated by arrows labeled Fli-1. S, supershifted Fli-1 nuclear-protein complex; A, nonspecific nucleoprotein complex.