Fig. 7. Fli-1 mRNA is unaffected by PMA treatment or blockade by PD98059. After overnight growth arrest, Dami cells were incubated with PD98059 (30 µ M) or SB202190 (500 nmol/liter) for 30 min before PMA (100 nmol/liter) treatment. Total RNA was harvested at 12 h, and Fli-1 mRNA expression was analyzed by Northern blot. Membranes were rehybridized with [32P]GAPDH cDNA to demonstrate the amount of RNA loaded.