Fig. 5. Fli-1 binds constitutively to the Ets motif in the GPIX promoter. A 32P-labeled oligonucleotide probe corresponding to the GPIX proximal promoter bearing the Ets binding site, GPIX-EBS, was incubated with nuclear extracts from starved and PMA-treated Dami cells in the presence of 100 x unlabeled competitors or antibodies as outlined in "Materials and Methods." The specific oligonucleotide-Fli-1 complexes are indicated by arrows labeled Fli-1. S, supershifted Fli-1 nuclear-protein complex; A, nonspecific nucleoprotein complex.