Fig. 1. PMA induces GPIX mRNA expression in Dami cells. The ability of PMA to modulate GPIX mRNA expression in Dami cells was initially evaluated by Northern blotting. Dami cells were growth-arrested overnight and incubated with PMA (100 nmol/liter). Total RNA was harvested at times indicated and subject to Northern blotting. Membranes were rehybridized with [32P]GAPDH cDNA to demonstrate the amount of RNA loaded. Results are representative of three independent experiments.