Fig. 9. Time course of generation of lipid peroxides in TNF-stimulated C2VC and C2/AS cells. Cells were stimulated with TNF (30 ng/ml) and homogenized at varying times to measure lipid peroxides. Lipid peroxides were measured using the thiobarbituric acid procedure, and standard curves were generated with malondialdehyde. Values shown are means ± SE for three separate experiments.