Fig. 8. Time course of IB degradation in TNF-stimulated C2VC and C2/AS cells. Cells were stimulated with TNF (30 ng/ml), and total cell proteins were isolated and solubilized at varying times. Samples were run on SDS-PAGE and blotted, and Western blots were probed with a monoclonal anti-IB antibody. Images shown are representative of those of three separate experiments.