Fig. 6. Effect of the oxidant H2O2 on viability of C2/CEP4 and C2/AS cells. Cells were used at 7 days after plating and injured for 30 min with various amounts of H2O2 in HBSS with Ca and MgCl2 for 30 min. The HBSS was replaced with phenol red-free DMEM with XTT reagents, and the conversion to the formazan dye was followed for 120 min. Values shown are means ± SE for three experiments. Control, 0.03 mM, 0.1mM; 0.3 mM 1mM, 3mM, .