Table 1 Summary of PTEN mutation effects on apoptosis and growth in SHEP neuroblastoma cells

PTEN construct Function of mutation Effect on apoptosis IGF rescue? Growth

PTP vector None-vector control Serum withdrawal and hyperosmotic-induced apoptosis Yes Normal
wtPTEN Overexpress PTEN Enhances serum withdrawal-induced apoptosis No Decreased
C124S Mutation in catalytic domain prevents phosphatase activity Same as PTP control Only from hyperosmotic-induced apoptosis Decreased
G129E Mutation in catalytic domain prevents lipid recognition and phosphatase activity Decreases apoptosis Yes Decreased