Fig. 4. Promoter III Oct-1 binding site function in melanoma cell lines. Melanoma cell lines were transiently transfected with a wild-type promoter III luciferase construct and a promoter III construct with a mutated Oct-1-DNA binding site. After 48 h, cells were harvested for luciferase activity assays. The wild-type luciferase counts were normalized to unity to display relative fold induction for the mutated promoter.