Fig. 4. Endogenous VDR expression in SW620 cells transfected with ZEB expression vector. SW620 cells were transiently transfected with 6 µg of control vector (Lanes 1 and 2) or 6 µg of ZEB expression vector (Lanes 3 and 4). Cells were harvested at 48 h after transfection. Total protein extraction and Western blot analyses were performed as indicated in "Materials and Methods." The experiment was repeated twice with duplicate samples, and a representative Western blot is shown.