Fig. 3. Cell-specific activation of the VDR promoter by ZEB. In A, HCT-116, SW620, and LNCaP cells were transiently transfected with 0.2 µg/well of VDR reporter gene or control vector pGL3Basic in the absence or presence of 0.5 µg of ZEB expression vector. The results represent the change in transcriptional activity in the VDR reporter gene in the presence of ZEB normalized to that of the pGL3Basic control vector. The average of three independent transfections and SDs are shown. In B, Northern blot analyses for the expression of ZEB, VDR, and actin were performed as described in "Materials and Methods" for LNCaP (Lane 1), HCT-116 (Lane 2), and SW620 (Lane 3) cells.