Fig. 8. Effect of Gab1 expression on EGF-dependent mitogenesis in 10W2T cells. 10W2T-none (clone 7), 10W2T-Gab1 (clone 1), and 10W+8 cells were serum-starved for 16 h and incubated with 1 µCi/well [3H]thymidine in the presence or absence of 50 ng/ml EGF at 37°C for 24 h. The radioactivity of the incorporated [3H]thymidine was measured. Exogenous Gab1 expression did not enhance the mitogenic activity of 10W2T cells in response to EGF stimulation. The data are the means ± SD of six determinations.