Fig. 5. Tumorigenic 10W2T cells lack Gab1 expression and fail to recruit SHP-2 to activated EGFR. 10W2T cells were treated with or without 100 ng/ml EGF at 37°C for 1 min. Cells were lysed, and the immunoprecipitation (IP) was performed with specific antibodies for SHP-2 (Lanes 3 and 4), Gab1 (Lanes 5 and 6), EGFR (Lanes 7 and 8), or with NRS as controls (Lanes 1 and 2). Samples were analyzed by SDS-PAGE and immunoblotting with anti-p-Tyr antibody, followed by ECL. The data shown are representative of three independent experiments.