Fig. 6. dn-JNK1 blocks caspase-8 activation in PKC/Ras-mediated apoptosis. After the treatment with either high-dose PMA (500 nM for 24 h) or an anti-Fas Ab (for 30 min), lysates from Jurkat/ras cells transfected with dn-JNK or pGEX were prepared. Coimmunoprecipitation of FADD with caspase-8 and immunoblotting for cleavage products of caspase-8 (p18) or BID (p15) were performed as described above.