Fig. 3. Caspase-8 activation during PKC/Ras- or Fas-mediated apoptosis. After either Fas ligation or chronic, high-dose PMA treatment, lysates containing equal amounts of total protein from Jurkat, Jurkat/Fasm, or Jurkat/FADDm cells without (upper two panels) or with (lower two panels) expression of v-ras were separated on a SDS-PAGE gel, transferred to nitrocellulose, and immunoblotted with the anti-caspase-8 Ab.