Fig. 2. Effect of RhoGDI on Rho family protein distribution in living cells. HeLa cells actively growing in medium containing 10% calf serum were transfected with plasmids containing GFP-tagged GTPase constructs in the absence or presence of a 3-fold excess of a RhoGDI expression construct by LipofectAMINE PLUS reagent in serum-free medium, and 6 h posttransfection, cells were maintained in medium containing 10% serum. At 24 h posttransfection, cells were viewed with a Zeiss Axiophot fluorescence microscope, and digitized images were recorded.