Fig. 1. Localization of TC10 in living mammalian cells. HeLa cells actively growing in medium containing 10% calf serum were transfected with plasmids containing GFP-tagged constructs by LipofectAMINE PLUS reagent in serum-free medium, and at 6 h posttransfection, cells were transferred into medium with 10% serum. At 24 h posttransfection, cells were viewed with a Zeiss Axiophot fluorescence microscope, and digitized images were recorded.