Fig. 8. Activation of short-prodomain caspase-6. IEC cytosol was processed as described above and analyzed for activation of short-prodomain caspase-6 by Western blot. Note the appearance of the subunit of caspase-6 (p17) after 60 min as the procaspase signal (p34) diminishes, showing that caspase-6 activation during anoikis is a late event during the apoptotic cascade.