Fig. 7. Translocation of activated caspase-3 during anoikis. IECs were harvested after 45 and 90 min during anoikis and analyzed for detection of activated caspase-3 by immunocytochemistry. Note the shift of brown staining for activated caspase-3 from a predominant cytosolic location (+45 min) to a the strong demarcation of apoptotic nuclear beads during late stages of apoptosis (+90 min).