Fig. 5. Caspase-8 shows delayed activation during anoikis. IECs were processed as described above. Caspase-8 (p48 and p45 isoforms) is preformed in IECs and remains initially inactivated during anoikis. Thirty min after loss of anchorage, the prodomain of caspase-8 is processed (yielding the p43 and p41 fragments), and the p16 subunit of activated caspase-8 is detectable after 4560 min.