Fig. 3. IECs are susceptible to anoikis independent of their state of differentiation. IECs were isolated from different regions along the crypt-lumen axis (CB, crypt base; MI, mid-crypt; LU, lumen region of crypt) and kept in suspension for 3 h to assess susceptibility to anoikis. As shown by DNA electrophoresis (A) and caspase-3-like activity (B), no difference can be detected between IECs of different developmental stages. Data are mean ± SD of three experiments. M, 100-bp ladder.