Fig. 2. After loss of cell anchorage, IECs rapidly undergo massive apoptosis. IECs were harvested and assessed for DNA content by PI staining. The cell aggregates remaining after a brief dispase treatment were identified and gated in the pulse width count. At the indicated times after loss of anchorage, PI staining of IECs was analyzed, showing a massive increase in the sub-G1 (M1) population of cells after 180 min undergoing anoikis, indicative of apoptosis.