Fig. 5. Expression of Bcl-2 and Bax in HC11 cells exposed to PhIP. Competent cultures of HC11 cells were treated with 100 µm PhIP or DMSO (control) for 48 h in the DM, and expression of Bcl-2 and Bax were examined immunocytochemically (A–D) and by Western blot analysis (E, F). A, Bcl-2 expression in control cells. B, Bcl-2 expression in PhIP-treated cells. C, Bax expression in control cells. D, Bax expression in PhIP-treated cells. E, Western blot analysis of Bcl-2. F, Western blot analysis of Bax. A–D, x200.