Fig. 4. Contribution of the AP-1 transcription factor to regulated activity of the CD11c promoter. Reh cells were transfected with the indicated reporter constructs and treated with bryo 1. For each construct, fold induction represents the ratio between the luciferase and Renilla activity produced in the presence of bryo 1. Each construct was assayed three times, and a representative experiment is shown. The wild-type and mutant AP-1 and Sp1 sites are depicted as and (AP-1) or (Sp1), respectively. The pCD11c160(-70mut)-Luc reporter construct is mutated at the Sp1-binding site at -70; the pCD11c160(-60mut)-Luc reporter construct is mutated at the AP-1-binding site at -60. The pCD11c160-Luc reporter construct contains the -160/+43 region of the promoter, whereas the pCD11c61-Luc reporter construct contains the region -61/+43 but lacks the Sp1-binding site at -70; the pXP2 construct is the promotorless control.