Fig. 2. Bryo 1 induced ERK/MAPK activity in the Reh cell line. In A, Western blots were run using total cell lysates from Reh cells treated with bryo 1 and probed with MoAbs specific for the phosphorylated p44/p42 MAPK. Bryo 1 is shown inducing the phosphorylated form of p42. In B, a kinase assay was run using phospho-p44/p42 MAPK immunoprecipitates, which was then incubated with Elk-1 substrate. Western blots were run and probed with antibodies specific for phopho-Elk-1. Each experiment was completed in duplicate with a representative experiment shown.